Mice cloned from skin cells
نویسندگان
چکیده
منابع مشابه
Mice cloned from embryonic stem cells.
Cloning allows the asexual reproduction of selected individuals such that the offspring have an essentially identical nuclear genome. Cloning by nuclear transfer thus far has been reported only with freshly isolated cells and cells from primary cultures. We previously reported a method of cloning mice from adult somatic cells after nuclear transfer by microinjection. Here, we apply this method ...
متن کاملMice cloned from induced pluripotent stem cells (iPSCs).
Differentiated somatic cells of various species can be reprogrammed into induced pluripotent stem cells (iPSCs) by ectopically expressing a combination of several transcription factors that are highly enriched in embryonic stem cells (ESCs). The generation of iPSCs in large animals has raised the possibility of producing genetically modified large animals through the nuclear transplantation app...
متن کاملAdult mice cloned from migrating primordial germ cells.
We previously reported that the genomes of gonadal germ cells at 11.5-19.5 days postcoitum (dpc) are incompetent to support full-term development of cloned mouse embryos. In this study, we performed nuclear transfer using primordial germ cells (PGCs) from earlier stages at 8.5-10.5 dpc. When PGC nuclei at 8.5, 9.5, and 10.5 dpc were transferred into enucleated oocytes, seven cloned embryos deve...
متن کاملMice cloned from white adipose tissue-derived cells.
Isolation of different cells from adipose tissue was performed according to the previous reports (Rodeheffer et al., 2008; Sugii et al., 2010). Briefly, the inguinal fat pads of adult male B6D2F1 (8-12 weeks of age) mice were harvested, washed several times with phosphate-buffered saline (PBS) and excised into small pieces. The tissues were digested with 0.1% type I collagenase (Sigma) for 50 m...
متن کاملproduction of cloned mice by nuclear transfer of cumulus cells
background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2007
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0611358104